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目的 采用生物信息学、网络药理学和机器学习技术探究桑色素治疗牙周炎的可能机制。方法 采用BATMAN-TCM 2.0、HERB等数据库收集桑色素靶点；通过Genecards、OMIM等数据库检索牙周炎相关基因，并结合GEO数据库筛选差异基因。免疫浸润分析牙周炎组织的炎症细胞表达。构建PPI网络、GO和KEGG富集，通过加权基因共表达网络和机器学习鉴定桑色素治疗牙周炎的核心靶标，利用分子对接验证桑色素与关键靶点的结合力。最后，采用体外细胞实验验证了桑色素改善炎症环境的作用机制。结果 共获得桑色素治疗牙周炎的潜在靶标72个。桑色素主要通过白细胞介素（IL）-17信号通路，作用于丝裂原活化蛋白激酶（MAPK）14和基质金属蛋白酶（MMP）-9等核心靶点治疗牙周炎。体外细胞实验表明，桑色素能够呈剂量依赖性抑制一氧化氮的释放以及肿瘤坏死因子（TNF-α）、IL-6、IL-1β炎症因子表达，这种抑制可能是通过抑制MAPK14、MMP-9表达调控IL-17通路改善炎症。结论 桑色素能够通过抑制MAPK14、MMP-9表达调控IL-17通路改善牙周炎。
Abstract：Objective This study employed bioinformatics, network pharmacology, and machine learning techniques to explore the possible mechanism of morin in the treatment of periodontitis. Methods Morin targets were collected using databases such as BATMAN-TCM 2.0 and HERB; The genes related to periodontitis were searched for using databases such as Genecards and OMIM, and the differential genes were screened in combination with the GEO database. Immune infiltration analysis of inflammatory cell expression in periodontitis tissues. Construct PPI networks and GO and KEGG enrichment. Weighted gene co-expression networks and machine learning were used to identify the core targets of morin in the treatment of periodontitis, and molecular docking was employed to verify the binding force between morin and key targets. Finally, in vitro cell experiments were conducted to verify the mechanism by which morin improves the inflammatory environment. Results A total of 72 potential targets of morin for the treatment of periodontitis were obtained. Morin mainly treats periodontitis through core targets such as the IL-17 signaling pathway, MAPK14 and MMP-9. In vitro experiments have shown that morin can dose-dependently inhibit the release of NO and the expression of inflammatory factors such as TNF-α, IL-6, and IL-1β. This inhibition may be achieved by regulating the IL-17 pathway and improving inflammation by inhibiting the expression of MAPK14 and MMP-9. Conclusion Morin can regulate the IL-17 pathway by inhibiting the expression of MAPK14 and MMP-9 to improve periodontitis.
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基本信息:
DOI：10.15956/j.cnki.chin.j.conserv.dent.2026.03.002
中图分类号:R781.42
引用信息:
[1]连冰洁,黄文,吴泽钰,等.基于生物信息学、机器学习和网络药理学探究桑色素治疗牙周炎的作用机制研究[J].牙体牙髓牙周病学杂志,2026,31(03):131-140.DOI:10.15956/j.cnki.chin.j.conserv.dent.2026.03.002.
基金信息:
新疆维吾尔自治区自然科学基金重点项目（2022D01D57）; 新疆维吾尔自治区研究生创新项目（XJ2024G175）; 新疆维吾尔自治区“天山英才”科技创新领军人才项目（2023TSYCLJ0032）
2025-11-01
2025
2025-12-05
2026-01-05
2026
1
2026-03-28
2026-03-28