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目的:探讨血清C1q/TNF相关蛋白3(CTRP3)、细胞因子样蛋白1(CYTL-1)在慢性牙周炎患者中的表达及其与牙周指标和炎症指标的相关性。方法:选取于2022年4月至2023年4月收治的慢性牙周炎患者90例列为牙周炎组,依据牙周情况分为轻度组(33例)、中重度组(57例),另选取同期至本院体检口腔健康者40例列为健康组。探诊牙周炎组的牙周指标:探诊深度(PD)、出血指数(BI)、菌斑指数(PLI)、附着丧失(AL)、牙龈指数(GI);ELISA法检测血清指标:白细胞介素-1 β(IL-1 β)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、CTRP3、CYTL-1。分析血清CTRP3、CYTL-1与炎症指标、牙周指标的相关性;比较CTRP3、CYTL-1诊断慢性牙周炎严重程度的价值。结果:牙周炎组的IL-1 β、IFN-γ、TNF-α高于健康组,CTRP3、CYTL-1低于健康组(P<0.05);中重度组的IL-1 β、IFN-γ、TNF-α、PD、BI、PLI、AL、GI高于轻度组,CTRP3、CYTL-1低于轻度组(P <0.05);CTRP3、CYTL-1与IL-1 β、IFN-γ、TNF-α、PD、BI、PLI、AL、GI呈负相关(P <0.05);血清CTRP3、CYTL-1联合诊断曲线下面积(AUC)优于两者单独诊断AUC(P<0.05)。结论:慢性牙周炎患者血清CTRP3、CYTL-1水平较低,与牙周指标和炎症指标有关,可用于诊断慢性牙周炎的严重程度,联合检测更具诊断价值。
Abstract：AIM:To investigate serum levels of C1q/TNF-related protein 3(CTRP3) and cytokine receptor like factor 1(CYTL-1) in patients with chronic periodontitis,and to assess their correlation with periodontal and inflammatory indicators.METHODS:90 patients with chronic periodontitis admitted to our hospital were enrolled as the periodontitis group.Based on the periodontal severity,they were classified into a mild group(33 cases) and a moderate to severe group(57 cases).Another 40 oral health patients who underwent physical examinations at our hospital during the same period were as the healthy control group.The periodontal indicators-including probing depth(PD),bleeding index,plaque index(PLI),attachment loss(AL),and gingival index-were recorded.Serum levels of Interleukin-1β,Interferon-γ(IFN-γ),Tumor necrosis Factor-α(TNF-α),CTRP3,and CYTL-1 were measured by enzyme-linked immunosorbent assay.The correlation between serum CTRP3、CYTL-1 and gingival crevicular fluid and periodontal indicators was analyzed;the value of CTRP3 and CYTL-1 in diagnosing the severity of chronic periodontitis was compared.RESULTS:Compared with the healthy control group,patients with chronic periodontitis had significantly higher serum levels of IL-1 β,IFN-γ,and TNF-α,and significantly lower levels of CTRP3 and CYTL-1(P<0.05).The moderate-to-severe group exhibited higher levels of inflammatory markers and worse periodontal parameters than the mild group(P<0.05),accompanied by lower CTRP3 and CYTL-1 concentrations(P<0.05).Serum CTRP3 and CYTL-1 levels were negatively correlated with IL-1 β,IFN-γ,TNF-α,PD,BI,PLI,AL,and GI(P<0.05).Combined detection of CTRP3 and CYTL-1 yielded a significantly greater area under the ROC curve(AUC) for diagnosing disease severity compared to either marker alone(P<0.05).CONCLUSION:The serum levels of CTRP3 and CYTL-1 are significantly decreased in patients with chronic periodontitis,and are related to periodontal status and systemic inflammation.They can be used to diagnose the severity of chronic periodontitis,and combined detection has more diagnostic value.
[1]边梦瑶,陈莉丽,雷利红.慢性牙周炎与帕金森病相关性的研究进展[J].浙江大学学报(医学版),2022,51(1):108-114.DOI:10.3724/zdxbyxb-2021-0111.
[2]刘莉,戈春城,黄婧.慢性牙周炎患者龈沟液中IL-35、IL-1 β及IFN-γ的表达与临床意义[J].分子诊断与治疗杂志,2022,14(9):1628-1631.DOI:10.3969/j.issn.1674-6929.2022.09.043.
[3]杨犇,常卫.慢性牙周炎患者龈沟液IL-6、TNF-α、MMP-8水平及临床意义[J].检验医学与临床,2020,17(14):2085-2087.DOI:10.3969/j.issn.1672-9455.2020.14.047.
[4]GUO S,MAO X,LIU J.Multi-faceted roles of C1q/TNF-related proteins family in atherosclerosis[J].Front Immunol,2023,14(1):125-128.DOI:10.3389/fimmu.2023.1253433.
[5]长孙梓音,雷王蕊,刘延庆,等.补体1q/肿瘤坏死因子相关蛋白(CTRP)家族在炎症相关疾病中的研究进展[J].细胞与分子免疫学杂志,2023,39(7):649-655.DOI:10.13423/j.cnki.cjcmi.009615.
[6]ZHU S,KUEK V,BENNETT S,et al.Protein Cytl1:Its role in chondrogenesis,cartilage homeostasis,and disease[J].Cell Mol Life Sci,2019,76(18):3515-3523.DOI:10.1007/s00018-019-03137-x.
[7]孟焕新.2018年牙周病和植体周病国际新分类简介[J].中华口腔医学杂志,2019,54(2):73-78.DOI:10.3760/cma.j.issn.1002-0098.2019.02.001.
[8]冯崇锦.口腔科疾病临床诊断与治疗方案[M].北京:科学技术文献出版社,2010:80-81.
[9]中华口腔医学会牙周病学专业委员会.重度牙周炎诊断标准及特殊人群牙周病治疗原则的中国专家共识[R].中华口腔医学杂志,2017,52(2):67-71.DOI:10.3760/cma.j.issn.1002-0098.2017.02.002.
[10]赵素倩,贾情,穆楠楠,等.益生菌联合新癀片对牙周病患者口腔致病菌群及龈沟液中骨代谢指标水平的影响[J].北京口腔医学,2023,31(6):419-423.DOI:10.20049/j.bjkqyx.1006-673X.2023.06.009.
[11]SALARI A,ALAVI F N.Effect of severity of chronic periodontitis on oral health-related quality of life[J].Maedica(Bucur),2021,16(2):239-245.DOI:10.26574/maedica.2021.16.2.239.
[12]王兴.第四次全国口腔健康流行病学调查报告[M].北京:人民卫生出版社,2018:106-113.
[13]GENCO R J,SANZ M.Clinical and public health implications of periodontal and systemic diseases:An overview[J].Periodontol 2000,2020,83(1):7-13.DOI:10.1111/prd.12344.
[14]PAPATHANASIOU E,CONTI P,CARINCI F,et al.IL-1 superfamily members and periodontal diseases[J].J Dent Res,2020,99(13):1425-1434.DOI:10.1177/0022034520945209.
[15]SHEIBAK N,HEIDARI Z,MAHMOUDZADEH-SAGHEB H.Immunoexpression of interferon-gamma in the interdental gingiva of chronic periodontitis patients with interferon-gamma(+874A/T)rs62559044 polymorphism[J].J Oral Biol Craniofac Res,2022,12(5):727-732.DOI:10.1016/j.jobcr.2022.08.019.
[16]ZHANG X,ZHU X,SUN W.Association between tumor necrosis factor-α(G-308A)polymorphism and chronic periodontitis,aggressive periodontitis,and peri-implantitis:A meta-analysis[J].J Evid Based Dent Pract,2021,21(3):10-18.DOI:10.1016/j.jebdp.2021.101528.
[17]MAJEED M M,AHMED I,ROOME T,et al.Association between interleukin-1 β gene polymorphism and chronic periodontitis[J].Eur J Dent,2021,15(4):702-706.DOI:10.1055/s-0041-1730041.
[18]ZHANG H,ZHANG-SUN Z Y,XUE C X,et al.CTRP family in diseases associated with inflammation and metabolism:Molecular mechanisms and clinical implication[J].Acta Pharmacol Sin,2023,44(4):710-725.DOI:10.1038/s41401-022-00991-7.
[19]SCHMID A,SCH?FFLER A,KARRASCH T.CTRP-3 regulates NOD1-mediated inflammation and NOD1 expression in adipocytes and adipose tissue[J].Inflammation,2021,44(6):2260-2269.DOI:10.1007/s10753-021-01497-w.
[20]LIN J,LIU Q,ZHANG H,et al.C1q/tumor necrosis factor-related protein-3 protects macrophages against LPS-induced lipid accumulation,inflammation and phenotype transition via PPAR y and TLR4-mediated pathways[J].Oncotarget,2017,8(47):82541-82557.DOI:10.18632/oncotarget.19657.
[21]LIN H,CHEN H,ZHAO X,et al.Advances in mesenchymal stem cell conditioned medium-mediated periodontal tissue regeneration[J].J Transl Med,2021,19(1):456-459.DOI:10.1186/s12967-021-03125-5.
[22]ZHU W,YANG X,LIU S,et al.The involvement of cytokine-like 1(Cytl1)in chondrogenesis and cartilage metabolism[J].Biochem Biophys Res Commun,2020,529(3):608-614.DOI:10.1016/j.bbrc.2020.06.069.
[23]SUN J S,WANG W W,LIAN H K.The clinical significance of changes in serum new cytokine CYTL1 in patients with knee osteoarthritis.[J]Int J Gen Med,2021,14(2):5105-5109.DOI:10.2147/IJGM.S322370.
[24]李超,徐紫薇,张璇,等.血清CCL21、CYTL-1水平与慢性牙周炎患者牙龈卟啉单胞菌感染及牙周临床指标的相关性[J].标记免疫分析与临床,2024,31(2):264-269.DOI:10.11748/bjmy.issn.1006-1703.2024.02.014.
基本信息:
DOI：10.15956/j.cnki.chin.j.conserv.dent.2025.07.003
中图分类号:R781.42
引用信息:
[1]李铄希,刘颖,董洋.慢性牙周炎患者血清CTRP3、CYTL-1水平及其临床意义[J].牙体牙髓牙周病学杂志,2025,30(07):387-391.DOI:10.15956/j.cnki.chin.j.conserv.dent.2025.07.003.
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